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| Name: |
| Fatma |
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Surname: |
Dogan Guzel |
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Institution: |
Ankara Yildirim Beyazit University: Ankara Yildirim Beyazit Universitesi |
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Type of Institution: |
University |
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ORCID number: |
https://orcid.org/0000-0001-7200-4615
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Google Scholar: |
https://scholar.google.com.tr/citations?user=BDG4qKwAAAAJ
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Publons: |
KDO-5399-2024
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Research Gate: |
https://www.researchgate.net/profile/Fatma-Dogan-G
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Country: |
Turkey
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WEB address: |
https://avesis.aybu.edu.tr/fdogan |
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Working groups of interest |
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| Keywords |
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Research field (Free keywords) |
nanopores, single molecule sensing, biosensors |
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| Materials/technologies |
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Scientific expertise |
Dr. Guzel received her PhD on nanopore studies, then moved to Jacobs University Bremen to carry out her PostDoctoral Studies on biological pores. During her post-studies, she focused on the single-channel characterization of bacterial porins (1,2). After returning to Turkiye as an independent PI, she set up her own electrophysiology and microfluidic/nanopore sensor laboratory by fundings received from national and international projects. In her laboratory, the main focus is on the development of on-chip antibiotic permeability sensors and understanding bacterial resistance mechanisms through porin proteins. 1. F. D. Güzel et al., Towards understanding single-channel characteristics of OccK8 purified from Pseudomonas aeruginosa, European Biophysics Journal, 50 (2021): 87-98. 2. F. D. Güzel, F. Çıtak, Development of an on-chip antibiotic permeability assay with single molecule detection capability, IEEE transactions on nanobioscience, 17/2 (2018): 155-160. |
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Capabilities |
Single-channel chracterization of such biological structures at single molecule is important because it provices exclusive information about the molecular passage through the pore, pore forming characteristics and susbtrate interactions, charge, and selectivity. Herein electrophysiology, the pore-forming peptides are placed in artificial lipid bilayers and ionic current through the pore is characterized at certain voltage and buffer conditions over time. The ionic current is resolvable down to 30 microseconds in time-domain and even more in frequency-domain signal. Dr. Guzel has also settings for forming giant unilamellar vesicles and studying pore-forming structures embedded in the membrane of the vesicles using other approaches (microfluidic sensings based on label-free impedance measurements). With that being said, Dr. Guzel will contribute to the action by potential single-channel characterization of pores and development of on-chip studies for potential production and characterization. |
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Relevant publications
(6 max.) |
| DOI https://doi.org/10.1039/C2CS35286A |
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| https://doi.org/10.1021/ac403391q |
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| https://doi.org/10.1049/mnl.2018.5206 |
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| https://doi.org/10.1109/JSEN.2018.2794781 |
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| https://doi.org/10.1042/BST20120121 |
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| https://link.springer.com/article/10.1007/s00249-021-01498-5 |
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Projects
(3 max.) |
| TUBITAK/BRITISH COUNCIL - A Fully-Integrated and Rapid On-Chip Antibiotic Susceptibility Test - A Case Study for Mycobacterium Tuberculosis |
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| TUBITAK - Characterisation of porins from hospital strains of Acinetobacter baumanni by single-channel electrophysiology |
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| IMI Translocation Project - Development of stable bilayer & robust reconstitution protocols for novel bilayers to allow a larger number of channels to be screened |
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